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الحصول على السعرMay 10, 2015 · Similar to a frequency table, but instead f: type cf: in the second line. For example: 10 20 30 40 50 60 70 80 cf: 5 13 20 32 60 80 90 100 The cumulative frequency is calculated by adding each frequency from a frequency distribution table to the sum of its predecessors. The last value will always be equal to the total for all observations since ...
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الحصول على السعرMay 22, 2012 · Reaction volumes will vary depending on the concentrations of the stock reagents. The final concentrations (CF) for a typical 50 μl reaction are as follows. X buffer (usually supplied by the manufacturer of the DNA polymerase; may contain 15 mM MgCl 2). Add 5 μl of 10X buffer per reaction. 200 μM dNTPs (50 μM of each of the four nucleotides).
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